Signalling

Part:BBa_K4161604:Design

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-09-27)


signalling peptide in L.lactis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Need to confirm the potency of the signaling peptide. Need to link the signalling peptide with downstream protein with neutral amino acids.


Source

It is a recombinant signaling peptide. The starting fragment comes from L.lactis genomic sequence. The last few sequences are artificial, which encodes LEISSTCDA, and is tested to be a potent signaling peptide compared to the natural one (Le Loir et.al, 2001).


References